Confirming gene modifications on release using Flow Cytometry assays
1:30 pm - Wednesday 2nd December 2026
- Measuring knock out knock in or knockdown expression as a functional way of confirming whether the gene modification occurred.
- Using PCR-based methods to assess genomic knock out or knock-in efficiency as an orthogonal measure on characterization.
- Reducing measurement of redundant attributes on release to prevent assay deviations in QC
